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AF6 gene on chromosome band 6q27 maps distal to the minimal region of deletion in epithelial ovarian cancer
Author(s) -
Saha Vaskar,
Lillington Debra M.,
Shelling Andrew N.,
Chaplin Tracy,
Yaspo MarieLaure,
Ganesan Trivadi S.,
Young Bryan D.
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140311
Subject(s) - cosmid , biology , microbiology and biotechnology , clone (java method) , complementary dna , gene , gene mapping , fluorescence in situ hybridization , breakpoint , chromosome , genetics
Chromosome 6 has been shown to contain at band q27 a minimal region of deletion associated with epithelial ovarian cancers and AF6 , a gene disrupted in acute myeloid leukemia with t(6;11)(q27;q23). Using rapid amplification of cDNA ends by polymerase chain reaction, the breakpoint in AF6 was confirmed and a cDNA clone identified. This clone was used as a probe to screen a chromosome 6 cosmid library, and a single cosmid C‐109F0645 was isolated. By fluorescence in situ hybridization, C‐109F0465 was found to map distal to the critically deleted region associated with ovarian malignancies. AF6 is therefore distinct from and lies telomeric to this region.