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Partial chromosome 21 amplification in a child with acute lymphoblastic leukemia
Author(s) -
Coniat Maryvonne Le,
Romana Serge P.,
Berger Roland
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140308
Subject(s) - biology , chromosome 21 , marker chromosome , monosomy , fluorescence in situ hybridization , chromosome , trisomy , microbiology and biotechnology , genetics , yeast artificial chromosome , karyotype , chromosome 22 , chromosomal translocation , gene mapping , gene
Monosomy 21 and metacentric markers corresponding in size to chromosomes 8 to 12 were found as the only clonal chromosomal changes in a child with acute lymphoblastic leukemia (ALL). Chromosome painting with a whole chromosome 21‐specific probe showed that the marker originated from chromosome 21. Fluorescence in situ hybridization with yeast artificial chromosome (YAC) probes to chromosome 21 showed genomic amplification with two, four, or more copies of the probed DNA sequences present on the marker. The most amplified regions of chromosome 21 were centromeric and telomeric to the Down's syndrome region. This observation supports the notion that amplification of only parts of chromosome 21 may be important in the leukemogenic process in spite of the high incidence of complete trisomy 21 in ALL.