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Normal human chromosome 2 induces cellular senescence in the human cervical carcinoma cell line SiHa
Author(s) -
Uejima Hiroshi,
Mitsuya Kohzoh,
Kugoh Hiroyuki,
Horikawa Izumi,
Oshimura Mitsuo
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140206
Subject(s) - biology , chromosome , senescence , microcell , gene , genetics , population , microbiology and biotechnology , medicine , environmental health , computer science , operating system
For identification of the chromosome carrying cellular senescence‐inducing activity, normal human chromosome 2, 3, 6, 7, 9, 11, or 12 tagged with a selectable marker gene ( neo ) was introduced into the human cervical carcinoma cell line SiHa via microcell‐mediated chromosome transfer. Seventy‐six percent (158/207) of the G418‐resistant clones obtained by the transfer of chromosome 2 showed a remarkable change in morphology (cells were flat), and 93% (147/158) of them ceased to divide (senesced) prior to 6–9 population doublings, whereas most of the clones generated by the transfer of other chromosomes exhibited a morphology similar to that of the parental cells and continued to grow. Chromosome analyses suggested that cells which escaped from senescence contained only a small fragment derived from the transferred chromosome 2, whereas the transferred chromosomes were apparently intact in most of the continuously growing microcell hybrids with introduction of other chromosomes. These results indicate that the normal human chromosome 2 carries a gene or genes that induce cellular senescence in SiHa cells.