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Localization of metastasis suppressor gene(s) for rat prostatic cancer to the long arm of human chromosome 10
Author(s) -
Nihei Naoki,
Ichikawa Tomohiko,
Kawana Youko,
Kuramochi Hiroaki,
Kugo Hiroyuki,
Oshimura Mitsuo,
Killary Ann M.,
RinkerSchaeffer Carrie W.,
Barrett J. Carl,
Isaacs John T.,
Shimazaki Jun
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140205
Subject(s) - metastasis suppressor gene , suppressor , metastasis , gene , chromosome , cancer research , biology , tumor suppressor gene , cancer , genetics , carcinogenesis
To examine the role of human chromosome 10 in development of prostatic cancer, we introduced human chromosome 10 into highly metastatic rat prostatic cancer cells by microcell‐mediated chromosome transfer. Microcell hybrid cells introduced with human chromosome 10 showed suppression of the metastatic ability to the lung to some extent without any suppression of tumorigenicity, although the tumor growth rate decreased slightly. To minimize the region that contains metastasis suppressive activity, the hybrid cells in metastasis foci of lung were established in culture and reanalyzed for portions of human chromosome 10 retained in the metastasis tissues. Cytogenetic and molecular analyses demonstrated that loss of the region between 10cen and D10S215 on human chromosome arm 10q was related to expression of the metastatic phenotype. These results demonstrate that the region between 10cen and D10S215 on human chromosome arm 10q contains at least one of the metastasis suppressor genes for rat prostatic cancer.