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Fluorescence in situ hybridization identifies new chromosomal changes involving 3q27 in non‐Hodgkin's lymphomas with BCL6 / LAZ3 rearrangement
Author(s) -
Wlodarska Iwona,
Mecucci Cristina,
Stul Michel,
Michaux Lucienne,
Pittaluga Stefania,
Hernandez Jesus Maria,
Cassiman JeanJacques,
De WolfPeeters Christiane,
van den Berghe Herman
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140102
Subject(s) - bcl6 , fluorescence in situ hybridization , chromosomal translocation , biology , lymphoma , gene rearrangement , breakpoint , follicular lymphoma , cytogenetics , cosmid , chromosome , pathology , in situ hybridization , microbiology and biotechnology , genetics , b cell , immunology , medicine , gene , antibody , gene expression , germinal center
Twelve B‐cell non‐Hodgkin's lymphomas with BCL6/LAZ3 rearrangement selected from a series of 30 lymphomas with cyto‐genetically detectable 3qter abnormalities were characterized at the histological, clinical, and cytogenetic levels, including fluorescence in situ hybridization (FISH) analysis, which was performed in all cases but one. A classical t(3;14) and t(3;22) were found in three patients (25%). In the remaining cases, eleven different 3q27 abnormalities were demonstrated and characterized with the use of chromosome painting. Seven of twelve “variant” rearrangements identified in our series affecting 1p32, 1p34, 3p14, 6q23, 12p13, 14q11, and 16p13 have not been reported before. Moreover, involvement of both homologs of chromosome 3 in distinct translocations was detected as an unexpected result in two cases and was confirmed via FISH in a third case. The putative bichromosomal rearrangements of the 3q27 region were evidenced by Southern analysis in one of these cases. In another case, FISH with a cosmid spanning the 3q27 breakpoint region demonstrated the involvement of BCL6/LAZ3 only in one of two t(3q27). In our series, which was selected on cytogenetic and molecular criteria, 50% (6 of 12) of cases with BCL6/LAZ3 rearrangement were diagnosed as diffuse, large B‐cell lymphomas (DLCL). Another 33% (4 of 12) of cases were diagnosed as follicular center lymphomas (FL), with t(14;18)/BCL2 rearrangement in all but one case. Furthermore, in three follicular lymphoma cases in which multiple samples were analyzed, the disease showed no evidence of histological progression during a follow‐up period of 3‐14 years. The present data show that rearrangement of BCL6 is not restricted to high‐grade malignancies as was previously suggested but can also occur in low‐grade lymphomas. In addition, the appearance of BCL6/LAZ3 rearrangement in follicular lymphomas is not related to disease progression.