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Loss of Chromosome 17 loci in prostate cancer detected by polymerase chain reaction quantitation of allelic markers
Author(s) -
Brothman Arthur R.,
Steele Michael R.,
Williams Briana J.,
Jones Emma,
Odelberg Shan,
Albertsen Hans M.,
Jorde Lynn B.,
Rohr L. Ralph,
Stephenson Robert A.
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870130408
Subject(s) - loss of heterozygosity , locus (genetics) , polymerase chain reaction , microsatellite , biology , prostate cancer , allele , microbiology and biotechnology , gene , chromosome , prostate , genetics , cancer research , pathology , cancer , medicine
Using a polymerase chain reaction/microsatellite marker system, we demonstrated that 6 of 22 (27%) clinical stage B (early) primary prostate tumors showed loss of heterozygosity at one or more of five loci on chromosome 17. The sensitivity of this study was increased by use of a Phosphorlmager and statistical analysis of replicate tumor‐normal DNA pairs. Two patients showed tumor‐specific interstitial loss at a locus in close proximity to the familial breast cancer gene BRCAI. These findings suggest that genes on the proximal long arm of chromosome I7 play a pivotal role in the early development of at least a subset of prostatic tumors. © 1995 Wiley‐Liss, Inc.