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Identification of two transcripts of AMLI/ETO ‐fused gene in t(8;21) leukemic cells and expression of wild‐type ETO gene in hematopoietic cells
Author(s) -
Era Takumi,
Asou Norio,
Kunisada Takahiro,
Yamasaki Hiroshi,
Asou Hiroya,
Kamada Nanao,
Nishikawa ShinIchi,
Yamaguchi Kazunari,
Takatsuki Kiyoshi
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870130105
Subject(s) - biology , haematopoiesis , gene , microbiology and biotechnology , zinc finger , gene isoform , breakpoint , chromosomal translocation , myeloid leukemia , gene expression , genetics , stem cell , cancer research , transcription factor
The t(8; 21) is a common chromosomal abnormality, preferentially associated with acute leukemia showing features of myeloid differentiation. Recently, two genes– AMLI which has a unique runt domain, and ETO ( MTG8 )–have been isolated from the chromosomal breakpoint. In this study, we isolated and identified two fused transcripts from a leukemic cell line carrying t(8; 21). AMLI and ETO were fused at the same position in these transcripts. One of the transcripts codes a unique domain, including two zinc finger domains and three proline‐ and one leucine‐rich region. The other transcript codes only for one proline‐ and leucine‐rich region but lacks zinc finger domains. We demonstrated by polymerase chain reaction (PCR) analysis that I) these two transcripts are consistently expressed in leukemic cells with t(8; 21) obtained from patients and 2) expression of AMLI was not restricted to the particular stage of hematopoietic differentiation but was present in all hematopoietic cells investigated. We also provide evidence that two wild types of ETO transcripts containing the region of the ETO gene in fused transcripts are expressed in hematopoietic cells from different lineages. The widespread expression of AMLI and ETO in hematopoietic cells suggests a fundamental role of these proteins in hematopoiesis. Furthermore, the differences in the carboxy termini of ETO may modulate the activity of fused proteins resulting from the chromosomal translocation t(8; 21). © 1995 Wiley‐Liss, Inc.