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Fusion of the EWS Gene to a DNA segment from 9q22‐31 in a human myxoid chondrosarcoma
Author(s) -
Gill Sandra,
McManus Aidan P.,
Crew A. Jayne,
Benjamin Harry,
Sheer Denise,
Gusterson Barry A.,
Pinkerton C. Ross,
Patel Ketan,
Cooper Dr.Colin S.,
Shipley Janet M.
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870120412
Subject(s) - cosmid , biology , fluorescence in situ hybridization , microbiology and biotechnology , southern blot , locus (genetics) , gene , chondrosarcoma , genetics , chromosome , pathology , medicine
Southern blot analyses revealed a rearrangement of the EWS gene in a skeletal human myxoid chondrosarcoma. Interphase fluorescence in situ hybridization (FISH) studies, using cosmid clones F7 and G9 that flank the EWS locus on 22q12, confirmed the presence of this EWS gene abnormality. Cloning the rearranged EWS DNA fragment and mapping by FISH demonstrated that the EWS gene is joined to DNA sequences localised in 9q22‐31. These findings are consistent with previous cytogenetic reports of a recurrent t(9;22)(q22‐31;q11‐12) in the myxoid variant of chondrosarcoma and reveal involvement of the EWS gene in a fourth type of human sarcoma.