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Breakpoints of burkitt's lymphoma t(8;22) translocations map within a distance of 300 kb downstream of MYC
Author(s) -
Zeidler Reinhard,
Lipp Martin,
Joos Stefan,
Delecluse HenriJacques,
Bornkamm Georg W.,
Klobeck Gustav,
Vuillaume Michele,
Lenoir Gilbert M.
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090408
Subject(s) - chromosomal translocation , breakpoint , microbiology and biotechnology , biology , fluorescence in situ hybridization , locus (genetics) , gene , lymphoma , chromosome , immunoglobulin heavy chain , genetics , immunology
The variant translocation t(8;22) in Burkitt's lymphoma (BL) cells joins band q24 of chromosome 8 distal to the proto‐oncogene MYC to the immunoglobulin lambda locus. The distribution of breakpoints on chromosome 8 of 11 cell lines with t(8;22) has been investigated by in situ fluorescence hybridization and pulsed‐field gel electrophoresis. We show that these chromosomal breakpoints generally fall within a region of about 300 kb 3′ of MYC and that at least 8 out of 11 affect the previously characterized transcriptional unit PVT1 . Comparable results were obtained in earlier experiments analyzing the variant t(2;8). Recently, in a series of BL cells carrying t(8;14), breakpoints upstream of MYC have been described at a similar distance. Therefore, our results suggest that deregulation of MYC by the immunoglobulin loci can occur at a distance of up to about 350 kbp of MYC. Genes Chrom Cancer 9:282‐287 (1994). © 1994 Wiley‐Liss, Inc.