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Recurrent cytogenetic abnormalities in squamous cell carcinomas of the head and neck region
Author(s) -
Van Dyke Daniel L.,
Worsham Maria J.,
Drumheller Timothy,
Benninger Michael S.,
Krause Charles J.,
Baker Shan R.,
Wolf Gregory T.,
Carey Thomas E.,
Tilley Barbara C.
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090308
Subject(s) - head and neck , medicine , basal cell , pathology , oncology , surgery
We characterized the breakpoints, gains, and losses of chromosome material in squamous cell carcinomas of the head and neck region from 29 patients. Cell lines were karyotyped in 1/3 of cases, direct preparations or early in vitro harvests in 1/3, and both in 1/3 of cases. GTG‐banding was employed in all cases, as were C‐banding and RBG‐ and AgNOR‐staining in most. Some tumors were near‐diploid and others near‐tetraploid, but many had mixed populations, with diploid, tetraploid, and octoploid subclones representing essentially the same karyotypic pattern. The most frequent changes were deletions. Losses affecting 3p13‐p24, 5q12‐q23, 8p22‐p23, 9p21 ‐p24, and 18q22‐q23 ranged in frequency from 40% to 60% of tumors. Loss of the short arm of the inactive X occurred in 70% of tumors from female patients, and loss or rearrangement of the Y occurred in 74% of tumors from male patients. Loss of 18q appeared to be associated with short survival, as did the presence of multiple deletions. There was gain (2‐5 extra copies) of 3q21 ‐qter, 5p, 7p, 8q, and 11q 13‐q23 in 28‐38% of tumors. Three tumors had an hsr involving 11q13‐q21. Gain of material at 11q13 is postulated to be associated with amplification of the PRADI/CCND gene at that locus. A translocation between proximal 1 p and either an acrocentric short arm or proximal 8p or 9p was observed in squamous cell carcinomas of the head and neck region but not in female genital tract tumors. No other abnormalities appeared to be site specific, suggesting a pattern of genetic evolution in squamous cell carcinoma that is independent of anatomic site. Genes Chrom Cancer 9:192‐206 (1994). © 1994 Wiley‐Liss, Inc.