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Numerical chromosomal aberrations in thyroid tumors detected by double fluorescence in situ hybridization
Author(s) -
Taruscio Domenica,
Ried Thomas,
Ward David C.,
Carcangiu Maria Luisa
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090306
Subject(s) - monosomy , trisomy , pathology , fluorescence in situ hybridization , biology , chromosome , aneuploidy , chromosome 7 (human) , cytogenetics , adenoma , microbiology and biotechnology , karyotype , medicine , genetics , gene
Double fluorescence in situ hybridization with DNA probes specific for the (peri)centromeric regions of chromosomes 3, 7, 9, 11, 12, 18, and X was performed on fresh isolated nuclei and frozen tissue sections prepared from 2 nodular hyperplasias, 2 adenomas, and 7 papillary carcinomas of the thyroid in order to detect numerical chromosomal changes. Numerical chromosomal aberrations were found in all malignant specimens examined. A consistent presence of at least two trisomies was detected in most cases, especially in the follicular variant specimens; the highest degree of trisomy was observed for chromosome 12. Isolated monosomies of moderate degree for different chromosomes were found in 1 adenoma and 2 papillary carcinomas. Severe monosomy of chromosome 9 was the only significant feature observed in the single metastatic papillary carcinoma. Genes Chrom Cancer 9:180‐185 (1994). © 1994 Wiley‐Liss, Inc.