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Deletion mapping of the short arm of chromosome 3 in human malignant mesothelioma
Author(s) -
Lu You Yong,
Cheng Jin Quan,
Testa Joseph R.,
Jhanwar Suresh C.
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090114
Subject(s) - loss of heterozygosity , mesothelioma , locus (genetics) , biology , malignancy , restriction fragment length polymorphism , genetics , polymerase chain reaction , primer (cosmetics) , allele , gene , cancer research , pathology , medicine , chemistry , organic chemistry
Previous cytogenetic investigations have revealed frequent deletions and ocher unbalanced structural rearrangements of 3p in human malignant mesothelioma. We have performed a restriction fragment length polymorphism analysis by using the polymerase chain reaction and primer sets for seven DNA markers to examine loss of heterozygosity (LOH) from 3p in 25 malignant mesotheliomas. Among 24 cases informative at one or more 3p loci, 15 (62.5%) exhibited LOH with at least one marker. Deletion mapping in these tumors indicates that the common region of chromosomal loss resides within band 3p21, in the vicinity of the D3F15S2 locus. These results suggest that allelic loss from 3p21 is a frequent Occurrence in malignant mesothelioma and that one or more putative tumor suppressor genes at this site contribute to the pathogenesis of this malignancy. Genes Chrom Cancer 9:76‐80 (1994). © 1994 Wiley‐Liss, Inc.