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Localization of amplified MYC gene sequences to double minute chromosomes in acute myelogenous leukemia
Author(s) -
Slovak Marilyn L.,
Ho Jennifer Pelkey,
Pettenati Mark J.,
Khan Aziz,
Douer Daniel,
Lal Shail,
Thomas Traweek S.
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090111
Subject(s) - microbiology and biotechnology , biology , karyotype , southern blot , exon , chronic myelogenous leukemia , abl , fluorescence in situ hybridization , gene , leukemia , chromosome , cytogenetics , complementary dna , genetics , cancer research , signal transduction , tyrosine kinase
Cytogenetic and molecular studies were performed on two dmin‐bearing acute myelogenous leukemia (FAB‐M2) samples. Both cases were characterized by complex karyotypes containing interstitial deletions of the long arm of chromosome 8 altering band 8q24.1, aberrations affecting the short arm of chromosome 17, and multiple double minute chromosomes (dmin). Using a 1.4 kb cDNA probe coding for the third exon of the MYC oncogene, DNA slot blots indicated MYC gene sequences were amplified in both samples. Fluorescence in situ hybridization using a 9.0 kb genomic probe for MYC was performed in one we and localized the amplified MYC gene sequences to the dmin. Neither patient achieved a complete remission using traditional induction chemotherapy. The complex karyology with amplification of MYC gene sequences appears to represent a poor prognostic subgroup of acute myelogenous leukemia. Genes Chrom Cancer 9:62‐67 (1994). © 1994 Wiley‐Liss, Inc.