Premium
Integration site of human papillomavirus type‐18 DNA in chromosome band 8q22.1 of C4‐1 cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences
Author(s) -
Gallego Marta I.,
Lazo Pedro A.,
Zimonjic Drazen B.,
Popescu Nicholas C.,
Dipaolo Joseph A.
Publication year - 1994
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870090106
Subject(s) - microbiology and biotechnology , biology , fluorescence in situ hybridization , dna , chromosome , gene , genetics
The C4‐1 cell line derived from a non‐keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus‐18 DNA. Fluorescence in situ hybridization of C4‐1 cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome originating from an 8q; 12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4‐1 and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells. Genes Chrom Cancer 9:28‐32 (1994). © 1994 Wiley‐Liss, Inc.