Integration site of human papillomavirus type‐18 DNA in chromosome band 8q22.1 of C4‐1 cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences | Zendy

Gallego Marta I. | Zendy; Lazo Pedro A. | Zendy; Zimonjic Drazen B. | Zendy; Popescu Nicholas C. | Zendy; Dipaolo Joseph A. | Zendy

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Integration site of human papillomavirus type‐18 DNA in chromosome band 8q22.1 of C4‐1 cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences

Author(s) -

Gallego Marta I.,

Lazo Pedro A.,

Zimonjic Drazen B.,

Popescu Nicholas C.,

Dipaolo Joseph A.

Publication year - 1994

Publication title -

genes, chromosomes and cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.754

H-Index - 119

eISSN - 1098-2264

pISSN - 1045-2257

DOI - 10.1002/gcc.2870090106

Subject(s) - microbiology and biotechnology , biology , fluorescence in situ hybridization , dna , chromosome , gene , genetics

The C4‐1 cell line derived from a non‐keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus‐18 DNA. Fluorescence in situ hybridization of C4‐1 cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome originating from an 8q; 12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4‐1 and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells. Genes Chrom Cancer 9:28‐32 (1994). © 1994 Wiley‐Liss, Inc.

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