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Analysis of deletions of the long arm of chromosome 11 in hematologic malignancies with fluorescence in situ hybridization
Author(s) -
Kobayashi Hirofumi,
Espinosa Rafael,
Fernald Anthony A.,
Begy Catherine,
Diaz Manuel O.,
Beau Michelle M. Le,
Rowley Janet D.
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870080407
Subject(s) - fluorescence in situ hybridization , biology , chromosome , gene , suppressor , cytogenetics , long arm , microbiology and biotechnology , in situ hybridization , tumor suppressor gene , fish <actinopterygii> , genetics , cancer research , carcinogenesis , gene expression , fishery
We studied samples containing deletions of the long arm of chromosome 11 (11 q) from patients with hematologic malignancies by using cytogenetic and fluorescence in situ hybridization (FISH) techniques. Cytogenetic analysis of 28 patients and of a cell line showed that all deletions included band 11q23. FISH analysis demonstrated that the proximal part of 11q23, including NCAM , was deleted in 13 of 15 patients and the cell line. Recurring chromosomal losses in human tumors have been regarded as evidence that the affected regions contain tumor‐suppressor genes. These results suggest that the putative tumor‐suppressor gene is proximal to the MLL gene which is also located in 11q23. © 1993 Wiley‐Liss, Inc.