Premium
Expanded range of 11q13 breakpoints with differing patterns of cyclin D1 expression in B‐cell malignancies
Author(s) -
Raynaud Sophie D.,
Bekri Soumeya,
Leroux Dominique,
Grosgeorge Josiane,
Klein Bernard,
Bastard Christian,
Gaudray Patrick,
Simon MariePierre
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870080204
Subject(s) - breakpoint , cyclin d1 , fluorescence in situ hybridization , biology , chromosomal translocation , locus (genetics) , metaphase , microbiology and biotechnology , gene , in situ hybridization , genetics , gene expression , cell cycle , chromosome
We have analyzed the BCL1 locus in a series of 24 B‐cell tumors and cell lines with rearrangements of 11q13 (mostly t(11;14)(q13;q32) translocations). Using Southern hybridization and/or fluorescence in situ hybridization (FISH) on metaphase chromosomes, we have not only confirmed the scattering of the breakpoints between the BCL1 locus and the cyclin DI gene ( CCND1 ), but also shown that some of the breakpoints could be as far as 500 kb on either side of the latter. Expression of CCND1 was not restricted to cases with t(11;14)(q13;q32), but was also associated with other 11q13 rearrangements, such as a t(8;11)(p22;q13). Whatever the alteration at 11q13, a correlation was observed between the expression of CCND1 and the presence of a breakpoint within 150 kb upstream of the gene. On the contrary, three samples, including a bona fide t(11;14) translocation and two cases with breakpoints located outside the BCL1‐CCND1 area, did not exhibit detectable levels of CCND1 transcripts. Our results raise the possibility that several discrete molecular events can take place at 11q13 in B‐cell malignancies. © 1993 Wiley‐Liss, Inc.