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Tissue culture loss of aneuploid cells from carcinomas of the prostate
Author(s) -
König Josée J.,
Teubel Wilma,
Van Dongen Jan Willem,
Hagemeijer Anne,
Romijn Johannes C.,
Schröder Fritz H.
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870080105
Subject(s) - ploidy , biology , karyotype , aneuploidy , flow cytometry , fluorescence in situ hybridization , chromosome , pathology , tissue culture , carcinoma , prostate , cell culture , in situ hybridization , microbiology and biotechnology , in vitro , cancer , genetics , gene , medicine , gene expression
The frequency of aneuploid cells in cultured prostate carcinoma specimens was investigated. Ploidy distribution of the original tissue was established by flow cytometry (FCM). Fluorescence in situ hybridization (FISH) of chromosome I was applied to directly isolated and cultured cells to investigate whether any modifications in the ploidy distribution of chromosome I took place during tissue culture. In six tumor specimens that were diploid by FCM and FISH, no differences were found in the ploidy distribution of chromosome I before and after tissue culture. In eight tumors that were aneuploid by FISH, the percentage of aneuploid nuclei was significantly reduced from 28.0 ± 15.0 (range 13ndash;59%) in uncultured cells to 9.1 ± 4.4 (range 4ndash;18%) after tissue culture. The reduction of aneuploid nuclei ranged from 44 to 85%, which means that the majority of the aneuploid cell populations that were observed in the original specimens were undetectable in cultured samples. This suggests a preferential growth of normal epithelial cells. The data presented can explain the high percentage of diploid karyotypes usually found in short‐term cultured prostate carcinoma specimens. © 1993 Wiley‐Liss, Inc.