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Deletion mapping of the short arm of chromosome 8 in non‐small cell lung carcinoma
Author(s) -
Ohata Hiroyuki,
Emi Mitsuru,
Fujiwara Yoshiyuki,
Higashino Kazuya,
Nakagawa Ken,
Futagami Reiko,
Tsuchiya Eiju,
Nakamura Yusuke
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870070204
Subject(s) - loss of heterozygosity , biology , restriction fragment length polymorphism , allele , chromosome , lung cancer , small cell carcinoma , carcinoma , deletion mapping , tumor suppressor gene , small cell lung carcinoma , genetics , cancer research , microbiology and biotechnology , gene , pathology , carcinogenesis , polymerase chain reaction , medicine
Frequent losses of heterozygosity observed at several chromosomal loci in primary lung cancers have indicated the existence of several tumor suppressor genes associated with this type of cancer. We have examined loss of heterozygosity on chromosomal arm 8p in 49 cases of non‐small cell lung carcinoma, using 14 restriction fragment length polymorphism markers. Of 42 cases informative with at least one marker, 21 showed allelic loss, including 15 of 32 adenocarcinomas and 5 of 9 squamous cell carcinomas. The frequency of allelic loss on 8p was similar at all clinical stages. Deletion mapping defined a single common region of deletion in these tumors within an 8 cM interval at 8p21.3–p22 flanked by the loci defined by cMSR‐32 and cC18–245. © 1993 Wiley‐Liss, Inc.