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Detection of somatic changes in human renal cell carcinomas with oligonucleotide probes specific for simple repeat motifs
Author(s) -
Bock Susanne,
Epplen Jörg T.,
NollPuchta Heidi,
Rotter Michael,
Höfler Heinz,
Block Thomas,
Hartung Rudolph,
Jakse Gerhard,
Wilmanns Wolfgang,
Petrides Petro E.
Publication year - 1993
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870060208
Subject(s) - haeiii , biology , somatic cell , microbiology and biotechnology , oligonucleotide , dna , restriction enzyme , genetics , restriction fragment length polymorphism , gene , polymerase chain reaction
The purpose of our study was to detect somatic changes in renal cell carcinoma by multilocus fingerprinting. DNA fingerprints were generated from the DNA of normal and malignant renal tissue samples of 29 patients with nonhereditary kidney carcinoma by using oligonucleotide probes specific for simple repeat motifs such as (GTG) 5 , (CA) 8 , (GACA) 4 , or (TTAGGG) 3 . Each probe resdered a typical fingerprint pattern, because it is specific with respect to the target regions recognized in the genome. The restriction enzymes used were Hinfl and H aeIII. Changed banding patterns were detected by using (GTG) 5 in 20% of the tumors, in 20% for (CA) 8 after Hinfl digestion, and in 10% after H aeIII digestion. Even more informative probes were (GACA) 4 , showing 70% changes after H aeIII digestion, and (TTAGGG) 3 , with 80% changes after digestion with either enzyme. Since the simple repeat motifs recognized by (GACA) 4 are localized on the short arms of the acrocentric chromosomes (13, 14, 15, 21, and 22), it is possible that sequences important for renal carcinogenesis are present in these regions. The observation of changes in regions to which (TTAGGG) 3 hybridizes points to an involvement of DNA elements in telomeric sequence related regions in human kidney tumor formation. © 1993 Wiley‐Liss, Inc.