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Molecular cloning and analysis of chromosome band 11q23 involved in leukaemia‐associated translocations
Author(s) -
Das Soma,
Kearney Lyndal,
Bower Mark,
Chaplin Tracy,
Riley John H.,
Anand Rakesh,
Young Bryan D.
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870050312
Subject(s) - chromosomal translocation , biology , breakpoint , genetics , yeast artificial chromosome , locus (genetics) , chromosome , clone (java method) , karyotype , chromosome band , microbiology and biotechnology , contig , gene mapping , dna , gene , genome
Three overlapping yeast artificial chromosomes (YACs) spanning a 780 kb region of DNA around the CD3 locus on chromosome 11 have been isolated and characterised. The individual cloned regions have been mapped by in situ hybridisation to chromosome band 11 q23, and a restriction enzyme map of this region has been constructed. The positions of these clones in relation to a series of leukaemia‐associated chromosomal translocations has also been determined. It was concluded that, although two clones lay entirely proximal to the breakpoints examined, the third clone (13HH4) encompassed the breakpoints for the translocations t(4;11), t(6;11), and t(9;11). The t(9;11) was observed in an acute myeloid leukaemia in a patient previously treated for an unrelated malignancy. It would thus appear that the breakpoints at chromosome band 11q23 occurring in therapy‐related leukaemias are in the same region as those found in adult and childhood acute leukaemias and may result from a common underlying mechanism.