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Cytogenetic and molecular genetic characterization of papillary thyroid carcinomas
Author(s) -
Sozzi Gabrielia,
Bongarzone Italia,
Miozzo Monica,
Cariani Claudia T.,
Mondellini Piera,
Calderone Carla,
Pilotti Silvana,
Pierotti Marco A.,
Porta Giuseppe Della
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870050307
Subject(s) - karyotype , biology , chromosomal rearrangement , thyroid carcinoma , chromosome , cytogenetics , abnormality , gene , papillary carcinoma , thyroid , chromosomal abnormality , pathogenesis , pathology , cancer research , genetics , medicine , immunology , psychiatry
A combined cytogeneticand molecular analysis was performed on 11 cases of papillary thyroid carcinoma. A simple karyotypic abnormality was detected in five tumors, whereas six had no apparent chromosome change. In four of five rearranged cases the presence of a specific chromosomal abnormality involving chromosome 10 (cases 1 and 2) and chromosome I (cases 3 and 4) was associated with the rearrangement of two protooncogenes: RET and NTRKI (formerly trk ), respectively, with different donor genes. Moreover, the chromosomal localization of the involved genes and the type of chromosomal change observed suggested that RET and NTRKI activation occurred by intrachromosomal rearrangements. The six cases with normal karyotype did not show RET or NTRKI activation. These findings suggest that a combined cytogenetic and molecular approach would be useful in understanding the pathogenesis of thyroid neoplasia.