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Clinical impact of chromosome I aberrations in neuroblastoma: A metaphase and interphase cytogenetic study
Author(s) -
Christiansen Holger,
Schestag Joachim,
Christiansen Nina M.,
Grzeschik KarlHeinz,
Lampert Fritz
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870050208
Subject(s) - metaphase , cytogenetics , interphase , karyotype , chromosome , neuroblastoma , biology , molecular cytogenetics , microbiology and biotechnology , genetics , cell culture , gene
Neuroblastoma tumors are characterized by aberrations of chromosome I. Rapid detection of these chromosomal aberrations at diagnosis could give important clues to outcome and therapy. We attempted to detect numerical and structural aberrations of chromosome I not only by classical metaphase cytogenetics but also by interphase cytogenetics in order to overcome difficulties of karyotyping due to diminished metaphase quality and quantity in primary neuroblastoma samples. Karyotypic changes of chromosome I in 53 primary neuroblastomas were evaluated. In addition, we successfully performed interphase cytogenetics using single and double in situ hybridization procedures with chromosome I ‐specific repetitive DNA probes on nuclei preparations obtained from 46 and 20 tumors, respectively. Polysomies of structurally normal chromosomes I were predominantly seen in tumors with good prognosis, whereas deletions of 1p material were nearly exclusively confined to progressive tumors. Numerical and structural chromosome I aberrations as studied by metaphase and interphase cytogenetics are thus valuable prognostic markers in neuroblastoma.