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Identification of a whole‐arm translocation by in situ hybridization with directly fluorochrome‐labeled probes in a myelodysplastic syndrome
Author(s) -
Bajalica Svetlana,
BrøindumNielsen Karen,
Sørensen AnneGrethe,
Pedersen Niels Tinggaård,
Heim Sverre
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870050206
Subject(s) - chromosomal translocation , centromere , fluorescence in situ hybridization , derivative chromosome , biology , karyotype , satellite dna , isochromosome , cytogenetics , in situ hybridization , chromosome , in situ , microbiology and biotechnology , hybridization probe , chromomycin a3 , long arm , genetics , dna , chemistry , gene , gene expression , organic chemistry
A case of myelodysplasia was found to have a complex bone marrow karyotype, involving an apparent whole‐arm translocation between 17q and 18q. The application of a simplified fluorescence in situ hybridization technique, using directly fluorochrome‐labeled centromere‐specific alpha‐satellite DNA probes, demonstrated the presence of sequences from both chromosomes 17 and 18 in the centromere of the derivative chromosome. This proves that a true whole‐arm translocation had occurred. The case exemplifies how in situ hybridization analysis can be used to resolve interpretation problems in cancer cytogenetics.