Premium
Characterization of the submicroscopic deletion in the small‐cell lung carcinoma (SCLC) cell line U2020
Author(s) -
Drabkin Harry A.,
Mendez Mike J.,
Rabbitts Pamela H.,
Varkony Terry,
Bergh Jonas,
Schlessinger Joseph,
Erickson Paul,
Gemmill Robert M.
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870050110
Subject(s) - locus (genetics) , microbiology and biotechnology , cell , gene , biology , genetics
The small‐cell lung carcinoma cell line U2020 contains a submicroscopic, homozygous deletion that removes a chromosomal segment within 3p13‐p14, including the locus D3S3. We have sublocalized 49 additional probes to the 3p13‐p14.2 region and have identified 7 new DNA markers that arise from within the U2020 deletion. The estimated size of the deletion, based on marker density, is approximately 4–5 megabases (Mb). Including D3S3, 7 of the 8 markers have been linked by pulsed‐field gel (PFG) electrophoresis over an area of approximately 2 Mb. Including the one unlinked marker, PFG analysis accounts for about 3 Mb of the region. The U2020 deletion appears confined to the 3p13‐p14.2 region and does not include the candidate tumor suppressor gene, protein‐tyrosine phosphatase gamma (PTPG).