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Chromosomal sublocalization of the 2;13 translocation breakpoint in alveolar rhabdomyosarcoma
Author(s) -
Shapiro David N.,
Valentine Marc B.,
Sublett Jack E.,
Sinclair Anne E.,
Thomas Look A.,
Tereba Alan M.,
Scheffer Hans,
Buys Charles H. C. M.
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870040309
Subject(s) - breakpoint , chromosomal translocation , alveolar rhabdomyosarcoma , genetics , rhabdomyosarcoma , biology , medicine , sarcoma , pathology , gene
A characteristic balanced reciprocal chromosomal translocation [t(2;13)(q35;q14)] has been identified in more than 50% of alveolar rhabdomyosarcomas. As the first step in characterization of the genes involved in this translocation, we constructed somatic cell hybrids that retained either the derivative chromosome 2 or the derivative chromosome 13 without a normal chromosome 13 homologue. Ten linked DNA probes known to be located within bands 13q13‐q14 were mapped relative to the breakpoint on chromosome 13, allowing localization of the breakpoint region between two loci separated by 5.5 cM. A long‐range restriction map extending approximately 2,300 kb around these loci failed to provide evidence of rearrangement. Additionally, we confirmed that the FMS ‐like tyrosine kinase gene (FLT) , previously localized to 13q12 by in situ hybridization, is located proximal to the breakpoint, and we demonstrated that FLT is not a target for disruption by this tumor‐specific translocation.