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Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple‐color fluorescence in situ hybridization
Author(s) -
Ried Thomas,
Lengauer Christoph,
Cremer Thomas,
Wiegant Joop,
Raap Anton K.,
Ploeg Mels Van Der,
Groitl Peter,
Lipp Martin
Publication year - 1992
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870040109
Subject(s) - breakpoint , fluorescence in situ hybridization , metaphase , biology , microbiology and biotechnology , interphase , plasmid , chromosomal translocation , southern blot , dna , genetics , chromosome , gene
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of this breakpoint in individual tumor cells. With this approach, tumor‐specific breakpoints in translocation chromosomes can be detected at all stages of the cell cycle with high specificity.