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The LCK Gene Is Involved in the t(1;7)(p34;q34) in the T‐Cell Acute Lymphoblastic Leukemia Derived Cell Line, HSB‐2
Author(s) -
Burnett Robert C.,
David JeanClaude,
Harden Alanna M.,
Le Beau Michelle M.,
Rowley Janet D.,
Diaz Manuel O.
Publication year - 1991
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870030608
Subject(s) - biology , microbiology and biotechnology , breakpoint , gene , locus (genetics) , genetics , promoter , coding region , untranslated region , chromosomal translocation , gene expression , rna
HSB‐2 is a cell line derived from a patient who had T‐cell acute lymphoblastic leukemia (T‐cell ALL) with a t( 1;7)(p34;q34). We used a genomic probe from the T‐cell receptor beta (TCRB) locus (7q34) to identify DNA rearrangements in HSB‐2. Two rearranged Bg/11 DNA fragments were cloned, and one of these clones was shown to contain the translocation breakpoint on the derivative chromosome 1 [der(1)]. We used a probe derived from this clone to isolate an unrearranged phage clone encompassing the breakpoint at 1 p34. The restriction map of this clone was compared to the published maps of known protooncogenes located at 1 p32–34. By restriction mapping, Southern blot analysis, and DNA sequencing we showed that the translocation breakpoint on chromosome 1 is located within the first intron of the LCK gene. The LCK gene codes for p56 lck , a member of the SRC family of cytoplasmic tyrosine protein kinases. There are two classes of LCK transcripts (type 1 and type 11), each expressed from a distinct promoter, and each having a unique 5′ untranslated region (UTR); the protein coding regions of the two classes are identical. The breakpoint in the t( 1;7) separates the two LCK promoters and juxtaposes the constant region of the TCRB locus with the proximal promoter and with the protein‐coding region of the LCK gene on the der(1) chromosome.