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Improved interpretation of complex chromosomal rearrangements by combined GTG banding and in situ suppression hybridization using chromosome‐specific libraries and cosmid probes
Author(s) -
Smit V. T. H. B. M.,
Wessels J. W.,
Mollevanger P.,
Dauwerse J. G.,
Van Vliet M.,
Beverstock G. C.,
Breuning M. H.,
Devilee P.,
Raap A. K.,
Cornelisse C. J.
Publication year - 1991
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870030402
Subject(s) - cosmid , biology , karyotype , comparative genomic hybridization , telomere , chromosome , metaphase , microbiology and biotechnology , hybridization probe , chromosomal translocation , dna–dna hybridization , genetics , in situ hybridization , cytogenetics , dna , gene , gene expression
Chromosome aberrations of a hypodiploid ovarian carcinoma cell line (modal chromosome number 38) having a complex karyotype were analyzed using biotinylated DNA library probes that specifically hybridize to chromosomes 3, 6, 7, 8, 11, 13, and 16 from telomere (pter) to telomere (qter). A series of cosmid probes localized to the short arm of chromosome 16 were used to further investigate one of the two aberrant chromosomes 16 present in this cell line. The competitive in situ suppression (CISS) hybridization of DNA‐libraries was mostly performed subsequent to GTG‐banding of the same metaphase cell in order to interpret the hybridization signals optimally. This combined approach made it possible to detect the origin of chromosomal material that could not be identified using GTG‐banding. Furthermore, the in situ hybridization techniques appeared to be helpful in the characterization of complex translocations and for accurate breakpoint determination.