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Molecular assignment of a translocation breakpoint in acute myeloid leukemia with t(8;21)
Author(s) -
Shimizu Kimiko,
Ichikawa Hitoshi,
Miyoshi Hiroyuki,
Ohki Misao,
Kobayashi Hirofumi,
Maseki Nobuo,
Kaneko Yasuhiko
Publication year - 1991
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870030302
Subject(s) - chromosomal translocation , breakpoint , clone (java method) , microbiology and biotechnology , biology , myeloid leukemia , chromosome , genetics , chromosome 21 , gene , cancer research
An 8;21 translocation is a common chromosome abnormality associated with acute myeloblastic leukemia with maturation (M2 of French‐American‐British (FAB) classification). We have isolated chromosome 21 Not l linking clones; pulsed field gel electrophoresis analysis with one clone (LL263) detected an altered fragment of Not l‐digested leukemic cell DNA carrying t(8;21). The altered fragment was shown to be produced by the 8;21 translocation. The breakpoint in chromosome 21 was located about 13 kb to 100 kb proximal to the LL263 Nod site. Because the LL263 clone has a CpG island and is a short distance from the breakpoint, the clone itself may be considered as a candidate for part of the t(8;21 ) associated gene.