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Direct pulsed field gel electrophoresis of Wilms' tumors shows that dna deletions in 11 p 13 are rare
Author(s) -
RoyerPokora Brigitte,
Ragg Susanne,
HecklÖstreicher Brigitte,
Held Manuela,
Loos Ursula,
Call Katherine,
Glaser Tom,
Housman David,
Saunders Grady,
Zabel Bernhard,
Williams Bryan,
Poustka Annemarie
Publication year - 1991
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870030203
Subject(s) - microbiology and biotechnology , biology , gene , point mutation , chromosome , deletion mapping , gel electrophoresis , wilms' tumor , dna , chromosomal translocation , karyotype , mutation , genetics
In order to search for small tumor‐specific deletions in 11 p 13 we analysed DNA isolated from 30 fresh Wilms' tumor (WT) samples with pulsed field gel electrophoresis. For these studies we have isolated new probes from the ends of several Nod fragments. Using these and previously described probes from 11 p 13 we first completed and extended the existing map of the 11 p 13 region. The analysis of the tumor material showed that (1) tumor‐specific deletions were very rare: one homozygous deletion out of 30 tumors analysed, (2) hemizygous deletions were not observed in any of the tumors. The homozygous deletion in one patient spans 220 kb and is composed of a tumor‐specific translocation associated with a deletion on one chromosome and a deletion of about 220 kb on the other chromosome at the same site. The WT‐33 Wilms' tumor candidate gene maps to this deleted segment. A small constitutional deletion of 1,300 kb was identified in a patient with WT and genital tract malformations. These results suggest that in the majority of sporadic WT loss of gene function is due to subtle alterations in the gene, e.g., point mutations or very small deletions.