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In situ hybridization ascertains the presence of a translocation t(6;11) in an acute monocytic leukemia
Author(s) -
Derré Josette,
Cherif Dorra,
Le Coniat Maryvonne,
Julier Cécile,
Berger Roland
Publication year - 1990
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870020413
Subject(s) - chromosomal translocation , monocytic leukemia , in situ , thp1 cell line , in situ hybridization , acute monocytic leukemia , leukemia , acute leukemia , chemistry , medicine , biology , immunology , biochemistry , cell culture , genetics , gene , organic chemistry , gene expression
Abstract In situ hybridization was performed in a case of acute monoblastic leukemia (FAB type M5b) with a rearrangement of the long arm of chromosome 11. Cytogenetic analysis after R‐ and G‐banding showed an apparent deletion of 11q with a breakpoint at 11q23, and a translocation t(6;11) was suspected in certain metaphases. In situ hybridization with a biotinylated cosmid probe hybridizing at 11q25 confirmed the translocation t(6;11)(q27;q23). Use of nonradioactive in situ hybridization techniques for more precise characterization of chromosomal rearrangements in malignant cells is emphasized.