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Chromosomal localization of the human myeloperoxidase gene by in situ hybridization using oligonucleotide probes
Author(s) -
Zaki Sharif R.,
Austin Garth E.,
Chan Wing C.,
Conaty Ann L.,
Trusler Suzanne,
Trappier Sam,
Lindsey Rusha B.,
Swan David C.
Publication year - 1990
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870020403
Subject(s) - in situ hybridization , oligonucleotide , myeloperoxidase , in situ , gene , oligomer restriction , microbiology and biotechnology , biology , computational biology , genetics , chemistry , gene expression , immunology , organic chemistry , inflammation
Oligonucleotide probes have been used to map the myeloperoxidase ( MPO ) gene locus to chromosome bands 17q21–22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72‐base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium‐labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.