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Use of fluorescent in situ hybridization to detect chromosomal rearrangements in somatic cell hybrids
Author(s) -
Giaccia Amato J.,
Evans James W.,
Brown J. Martin
Publication year - 1990
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870020314
Subject(s) - biology , human genome , genome , in situ hybridization , fluorescence in situ hybridization , chromosome , comparative genomic hybridization , genetics , dna , somatic cell , microbiology and biotechnology , gene , gene expression
Abstract In situ hybridization of hamster/human hybrids with biotinylated human genomic DNA has revealed that human chromosomal DNA can integrate into the hamster genome and is not always cytologically detectable. This finding helps to explain why discordancy can arise in gene mapping by failing to recognize small pieces of foreign DNA in the rodent genome. Fluorescent in situ hybridization allows one to locate these fragments in rodent chromosomes visually and possibly to identify their chromosome of origin.