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The 11q13 amplicon of a mammary carcinoma cell line
Author(s) -
Lafage Marina,
Nguyen Catherine,
Szepetowski Pierre,
Pébusque MarieJosèphe,
Simonetti Jacqueline,
Courtois Geneviéve,
Gaudray Patrick,
Delapeyriere Odile,
Jordan Bertrand,
Birnbaum Daniel
Publication year - 1990
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870020302
Subject(s) - amplicon , locus (genetics) , biology , gene duplication , carcinoma , gene , fibroblast , chromosomal region , chromosome , cell culture , microbiology and biotechnology , genetics , polymerase chain reaction
Abstract Fifteen to 20% of breast carcinomas show amplification of genes located at 11q13. The HST/FGFK and INT2 fibroblast growth factor (FGF)‐related genes and the BCL1 locus are usually present in the amplification units. We have investigated the structure and chromosomal location of the 11q13 amplicon of the MDA‐MB‐134 mammary carcinoma cell line by using in situ chromosomal and pulsed field gel hybridizations. The results indicate that a limited number of amplification units are involved in the constitution of an extended chromosomal region located on 11q. These units do not show any important rearrangement over rather large distances around the HST/INT2 and BCL1 loci.