Premium
Suggestive evidence that genes controlling invasion and metastasis of T‐cell lymphomas are located on mouse chromosome 3
Author(s) -
Verschaeve Luc,
Verschueren Hendrik,
Vandendriessche Thierry,
van Hecke Dominique,
Verhaegen Steven,
de Baetselier Patrick
Publication year - 1989
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870010203
Subject(s) - biology , marker chromosome , karyotype , chromosome , gene , genetic marker , marker gene , metastasis , microbiology and biotechnology , cell culture , cell , cancer research , genetics , cancer
Cell lines differing in their malignant potential have been derived from the murine BW5147 T‐cell lymphosarcoma. To evalute the involvement of chromosomal aberrations in tumor progression within this model, we have analyzed the karyotypes and the in vitro invasiveness of 13 related nonmetastatic and metastatic variants. Giemsa banding revealed the presence of several marker chromosomes, one of which was of particular importance. Depending on the cell line, four variants of this marker I were found: Marker Ia corresponds to two translocated chromosomes 3, marker Ib is a deleted Ia marker, marker Ic is a Ib translocated to a small unidentified chromosome fragment, and marker Id is a further deleted Ib marker. The Ia and Id markers were characteristic for the noninvasive, nonmetastatic lines, whereas the Ib and Ic markers predominated in the invasive, metastatic variants. The results suggest that metastasis‐enhancing genes are located between the D and F1 band of mouse chromosome 3 and that metastasis‐suppressing genes are located between the F1 and H band of the same chromosome.