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An (8;14)(q24;q11) translocation involving the T‐cell receptor α‐chain gene and the MYC oncogene 3′ region in a B‐cell lymphoma
Author(s) -
Park James K.,
McKeithan Timothy W.,
le Beau Michelle M.,
Bitter Mitchell A.,
Franklin Wilbur A.,
Rowley Janet D.,
Diaz Manuel O.
Publication year - 1989
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870010105
Subject(s) - biology , chromosomal translocation , breakpoint , microbiology and biotechnology , genetics , gene rearrangement , breakpoint cluster region , locus (genetics) , gene , exon , recombinase , recombination signal sequences , recombination activating gene , recombination
We describe a t(8;14)(q24;q11) involving the T‐cell receptor α‐chain gene ( TCRA ) and the 3′ region of the MYC protooncogene in a B‐cell lymphoma. The B‐cell origin of this tumor was determined by its histological architecture, by immunophenotypic analysis, and by Southern analysis of immunoglobulin gene rearrangements. An identical fragment encompassing the translocation breakpoint junction was detected through Southern analysis using both a TCRA J and a MYC probe. The other alleles at the TCRA J and MYC loci were in the germline configuration. Restriction enzyme and nucleotide sequencing analyses revealed that the breakpoint junction on chromosome 8 lies approximately 700 base pairs (bp) downstream of the 3′ end of the third MYC exon; on chromosome 14, the break is located 12.6 kilobases (kb) downstream of the 3′ end of the Cδ fourth exon. A heptamer‐like consensus sequence on chromosome 14 adjacent to the translocation breakpoint implies the involvement of recombinase activity. However, no consensus sequences were found on chromosome 8 within 140 bp in either direction from the breakpoint. It is possible that this translocation involving MYC occurred during an attempt at an inappropriate rearrangement of the TCRA locus in a cell of B‐cell lineage.