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Small RNA sequencing of sessile serrated polyps identifies microRNA profile associated with colon cancer
Author(s) -
Kanth Priyanka,
Hazel Mark W.,
Boucher Kenneth M.,
Yang Zhihong,
Wang Li,
Bronner Mary P.,
Boylan Katherine E.,
Burt Randall W.,
Westover Michelle,
Neklason Deborah W.,
Delker Don A.
Publication year - 2019
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.22686
Subject(s) - microrna , small rna , colorectal cancer , rna , biology , circular rna , hyperplastic polyp , gene , cancer research , non coding rna , cancer , genetics , colonoscopy
Sessile serrated adenoma/polyps (SSA/Ps) of the colon account for 20–30% of all colon cancers. Small non‐coding RNAs, including microRNAs (miRNAs), may function as oncogenes or tumor suppressor genes involved in cancer development. Small RNA sequencing (RNA‐seq) was used to characterize miRNA profiles in SSA/Ps, hyperplastic polyps (HPs), adenomatous polyps and paired uninvolved colon. Our 108 small RNA‐seq samples' results were compared to small RNA‐seq data from 212 colon cancers from the Cancer Genome Atlas. Twenty‐three and six miRNAs were differentially expressed in SSA/Ps compared to paired uninvolved colon and HPs, respectively. Differential expression of MIR31 ‐5p, MIR135B ‐5p and MIR378A ‐5p was confirmed by RT‐qPCR. SSA/P‐specific miRNAs are similarly expressed in colon cancers containing genomic aberrations described in serrated cancers. Correlation of miRNA expression with consensus molecular subtypes suggests more than one subtype is associated with the serrated neoplasia pathway. Canonical pathway analysis suggests many of these miRNAs target growth factor signaling pathways.