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Sequential analysis of 18 genes in polycythemia vera and essential thrombocythemia reveals an association between mutational status and clinical outcome
Author(s) -
Luque Paz Damien,
Chauveau Aurélie,
Boyer Françoise,
Buors Caroline,
Samaison Laura,
Cottin Laurane,
Seegers Valérie,
Férec Claude,
Le Maréchal Cédric,
Gueguen Paul,
Lippert Eric,
Ianotto JeanChristophe,
Ugo Valérie
Publication year - 2017
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.22437
Subject(s) - essential thrombocythemia , polycythemia vera , gene , medicine , association (psychology) , genetics , oncology , biology , psychology , psychotherapist
Philadelphia‐negative classical myeloproliferative neoplasms (MPN) are clonal diseases characterized by driver mutations of JAK2 , MPL , or CALR . Additional mutations may occur in epigenetic regulators, signaling, or splicing genes that may be useful in the prognostic assessment of MPN patients. In primary myelofibrosis, molecular‐based prognostic scoring systems have been recently proposed, but few data are available to date for polycythemia vera (PV) and essential thrombocythemia (ET). In this study, we used a next generation sequencing‐based 18‐gene panel in 50 JAK2 V617F positive PV and JAK2 V617F positive ET patients from an institutional cohort investigated at diagnosis and at 3‐year follow‐up (3y). Disease progression at 3y was defined by a composite criterion. Patients (28 PV and 22 ET) were included according to their clinical status, with or without disease progression. At diagnosis, we found 28 additional mutations in 21 of the 50 patients. Patients with disease progression were more likely to have at least one additional mutation. There was no difference between PV and ET. All patients with two or more additional mutations exhibited disease progression at 3y. No novel mutations appeared at 3y. The allele burden increase by at least one mutation at 3y was more frequent in patients with disease progression. Our data suggest that screening for additional mutations in PV and ET could identify patients at a higher risk of disease progression. © 2017 Wiley Periodicals, Inc.