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Cell cultures in uterine leiomyomas: Rapid disappearance of cells carrying MED12 mutations
Author(s) -
Nadine Markowski Dominique,
Tadayyon Mahboobeh,
Bartnitzke Sabine,
Belge Gazanfer,
Maria Helmke Burkhard,
Bullerdiek Jörn
Publication year - 2014
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.22142
Subject(s) - biology , hmga2 , population , in vitro , transcriptome , mutation , gene , point mutation , genetics , cancer research , microbiology and biotechnology , pathology , gene expression , medicine , microrna , environmental health
Uterine leiomyomas (UL) are the most frequent symptomatic human tumors. Nevertheless, their molecular pathogenesis is not yet fully understood. To learn more about the biology of these common neoplasms and their response to treatment, cell cultures derived from UL are a frequently used model system, but until recently appropriate genetic markers confirming their origin from the tumor cell population were lacking for most UL, i.e., those not displaying karyotypic abnormalities. The identification of MED12 mutations in the majority of UL makes it possible to trace the tumor cell population during in vitro passaging in the absence of cytogenetic abnormalities. The present study is addressing the in vitro survival of cells carrying MED12 mutations and its association with karyotypic alterations. The results challenge numerous in vitro studies into the biology and behavior of leiomyomas. Cells of one genetic subtype of UL, i.e., those with rearrangements of the high mobility AT‐hook 2 protein gene ( HMGA2 ), seem to be able to proliferate in vitro for many passages whereas tumor cells from the much more frequent MED12 ‐mutated lesions barely survive even the first passages. Apparently, for the most frequent type of human UL no good in vitro model seems to exist because cells do not survive culturing. On the other hand, this inability may point to an Achilles' heel of this type of UL. © 2014 Wiley Periodicals, Inc.