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The leucine twenty homeobox ( LEUTX ) gene, which lacks a histone acetyltransferase domain, is fused to KAT6A in therapy‐related acute myeloid leukemia with t(8;19)(p11;q13)
Author(s) -
Chinen Yoshiaki,
Taki Tomohiko,
Tsutsumi Yasuhiko,
Kobayashi Satoru,
Matsumoto Yosuke,
Sakamoto Natsumi,
Kuroda Junya,
Horiike Shigeo,
Nishida Kazuhiro,
Ohno Hirofumi,
Uike Naokuni,
Taniwaki Masafumi
Publication year - 2014
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.22140
Subject(s) - homeobox , biology , gene , fusion protein , myeloid leukemia , runx1t1 , homeobox a1 , genetics , fusion gene , zinc finger , histone acetyltransferase , microbiology and biotechnology , gene expression , cancer research , transcription factor , recombinant dna
The monocytic leukemia zinc finger protein KAT6A (formerly MOZ ) gene is recurrently rearranged by chromosomal translocations in acute myeloid leukemia (AML). KAT6A is known to be fused to several genes, all of which have histone acetyltransferase (HAT) activity and interact with a number of transcription factors as a transcriptional coactivator. The present study shows that the leucine twenty homeobox ( LEUTX ) gene on 19q13 is fused to the KAT6A gene on 8p11 in a therapy‐related AML with t(8;19)(p11;q13) using the cDNA bubble PCR method. The fusion transcripts contained 83 nucleotides upstream of the first ATG of LEUTX and are presumed to create in‐frame fusion proteins. LEUTX is known to have a homeobox domain. Expression of the LEUTX gene was only detected in placenta RNA by RT‐PCR, but not in any tissues by Northern blot analysis. The putative LEUTX protein does not contain any HAT domain, and this is the first study to report that KAT6A can fuse to the homeobox gene. The current study, with identification of a new partner gene to KAT6A in a therapy‐related AML, does not elucidate the mechanisms of leukemogenesis in KAT6A‐ related AML but describes a new gene with a different putative function. © 2014 Wiley Periodicals, Inc.