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Characterization of chromosome 11 breakpoints and the areas of deletion and amplification in patients with newly diagnosed acute myeloid leukemia
Author(s) -
Sarova Iveta,
Brezinova Jana,
Zemanova Zuzana,
Bystricka Dagmar,
Krejcik Zdenek,
Soukup Petr,
Vydra Jan,
Cermak Jaroslav,
Jonasova Anna,
Michalova Kyra
Publication year - 2013
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.22058
Subject(s) - breakpoint , chromosome , myeloid leukemia , biology , fluorescence in situ hybridization , karyotype , chromosome regions , genetics , comparative genomic hybridization , microbiology and biotechnology , myeloid , cancer research , gene
Chromosome 11 abnormalities are found in many hematological malignancies. In acute myeloid leukemia (AML), a proto‐oncogene MLL (11q23.3) is frequently altered. However, rearrangements involving other regions of chromosome 11 have been reported. Therefore, we have characterized the chromosome 11 breakpoints and common deleted and amplified areas in the bone marrow or peripheral blood cells of newly diagnosed patients with AML. Using molecular–cytogenetic methods (multicolor fluorescence in situ hybridization (mFISH), multicolor banding (mBAND), microarrays, and FISH with bacterial artificial chromosome (BAC) probes, chromosome 11 abnormalities were delineated in 54 out of 300 (18%) newly diagnosed AML patients. At least 36 different chromosome 11 breakpoints were identified; two were recurrent (11p15.4 in the NUP98 gene and 11q23.3 in the MLL gene), and three were possibly nonrandom: 11p13 (ch11:29.31‐31.80 Mb), 11p12 (ch11:36.75‐37.49 Mb) and 11q13.2 (68.31‐68.52 Mb). One new MLL gene rearrangement is also described. No commonly deleted region of chromosome 11 was identified. However, some regions were affected more often: 11pter‐11p15.5 ( n = 4; ch11:0‐3.52 Mb), 11p14.1‐11p13 ( n = 4; ch11:28.00‐31.00 Mb) and 11p13 ( n = 4; ch11:31.00‐31.50 Mb). One commonly duplicated (3 copies) region was identified in chromosomal band 11q23.3‐11q24 ( n = 9; ch11:118.35‐125.00 Mb). In all eight cases of 11q amplification (>3 copies), only the 5′ part of the MLL gene was affected. This study highlights several chromosome 11 loci that might be important for the leukemogeneic process in AML. © 2013 Wiley Periodicals ,Inc.