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YAP1 and VGLL3 , encoding two cofactors of TEAD transcription factors, are amplified and overexpressed in a subset of soft tissue sarcomas
Author(s) -
HéliasRodzewicz Zofia,
Pérot Gaëlle,
Chibon Frédéric,
Ferreira Céline,
Lagarde Pauline,
Terrier Philippe,
Coindre JeanMichel,
Aurias Alain
Publication year - 2010
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20825
Subject(s) - amplicon , carcinogenesis , biology , yap1 , gene , phenotype , transcriptome , genetics , transcription factor , transcription (linguistics) , cancer research , microbiology and biotechnology , gene expression , polymerase chain reaction , linguistics , philosophy
In a series of 404 adult soft tissue sarcomas, analyzed by array‐CGH, we have observed in approximately 10% of them a genomic amplification of either chromosome bands 11q22 or 3p12. These two amplicons likely target the YAP1 and VGLL3 genes, respectively. Both genes encode proteins that are cofactors of the TEAD family of transcription factors. Very good correlations between amplification and expression levels were observed. Welch test analyses of transcriptome data demonstrate that tumors with amplicons share a large set of upregulated and downregulated genes. Inhibition of YAP1 and VGLL3 in cell lines with these amplifications/overexpressions leads to similar phenotypes: decrease of proliferation rate, and to a lesser extent decrease of migration properties. These data, and the fact that these amplicons are observed either in dedifferentited liposarcomas or in undifferentiated pleomorphic sarcomas, suggest that these genetics events could be involved in oncogenesis and progression of soft tissue sarcomas. © 2010 Wiley‐Liss, Inc.