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miR‐29b and miR‐125a regulate podoplanin and suppress invasion in glioblastoma
Author(s) -
Cortez Maria Angelica,
Nicoloso Milena Sabrina,
Shimizu Masayoshi,
Rossi Simona,
Gopisetty Gopal,
Molina Jennifer R.,
Carlotti Carlos,
Tirapelli Daniela,
Neder Luciano,
Brassesco Maria Sol,
Scrideli Carlos Alberto,
Tone Luiz Gonzaga,
Georgescu MariaMagdalena,
Zhang Wei,
Puduvalli Vinay,
Calin George Adrian
Publication year - 2010
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20808
Subject(s) - podoplanin , glioblastoma , cancer research , biology , medicine , immunohistochemistry
Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation and invasion. Recently, it was demonstrated that podoplanin membrane sialo‐glycoprotein encoded by PDPN gene is over‐expressed and related to cellular invasion in astrocytic tumors; however the mechanisms of regulation are still unknown. MicroRNAs are noncoding RNAs that regulate gene expression and several biological processes and diseases, including cancer. Nevertheless, their roles in invasion, proliferation, and apoptosis of glioblastoma are not completely understood. In this study, we focused on miR‐29b and miR‐125a , which were predicted to regulate PDPN , and demonstrated that these microRNAs directly target the 3′ untranslated region of PDPN and inhibit invasion, apoptosis, and proliferation of glioblastomas. Furthermore, we report that miR‐29b and miR‐125a are downregulated in glioblastomas and also in CD133‐positive cells. Taken together, these results suggest that miR‐29b and miR‐125a represent potential therapeutic targets in glioblastoma. © 2010 Wiley‐Liss, Inc.