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The use of tetradecanoylphorbol acetate‐stimulated peripheral blood cells enhances the prognostic value of interphase fluorescence in situ hybridization in patients with chronic lymphocytic leukemia
Author(s) -
Delgado Julio,
Aventin Anna,
Briones Javier,
Sanchez Jana,
Nomdedeu Josep,
Sierra Jorge
Publication year - 2010
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20744
Subject(s) - peripheral blood mononuclear cell , fluorescence in situ hybridization , chronic lymphocytic leukemia , fish <actinopterygii> , interphase , in situ hybridization , in situ , pathology , biology , medicine , microbiology and biotechnology , leukemia , gastroenterology , immunology , chemistry , gene expression , biochemistry , in vitro , gene , genetics , organic chemistry , fishery , chromosome
Interphase fluorescence in situ hybridization (I‐FISH) studies have a remarkable prognostic value in patients with chronic lymphocytic leukemia (CLL). I‐FISH studies can be performed either on tetradecanoylphorbol acetate stimulated peripheral blood cells (I‐FISH‐TPA) or unstimulated peripheral blood mononuclear cells (I‐FISH‐PBMC). The aim of the study was to evaluate whether this finding was clinically relevant in a group of 235 patients with CLL. Fifty‐six patients had both I‐FISH‐TPA and I‐FISH‐PBMC results. Compared with uncultured cells, the cytogenetic detection rate rose from 57 to 80% with the use of TPA‐stimulated cells ( P = 0.014). I‐FISH‐TPA provided a better prediction of treatment‐free survival compared with I‐FISH‐PBMC ( P = 0.031 vs. 0.166). Then, I‐FISH‐PBMC results from 93 historical patients were compared with 86 recent patients with I‐FISH‐TPA results. Genomic aberrations were detected in 46 and 67% of patients from the I‐FISH‐PBMC and I‐FISH‐TPA cohorts, respectively. The detection rate of 13q deletion as the only aberration increased from 10% with I‐FISH‐PBMC to 37% with I‐FISH‐TPA ( P = 0.006). In conclusion, I‐FISH‐TPA increased the detection rate of 13q deletion and had an improved prognostic value compared with I‐FISH‐PBMC. © 2009 Wiley‐Liss, Inc.