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Microarray‐based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status
Author(s) -
Schwaenen Carsten,
Viardot Andreas,
Berger Hilmar,
Barth Thomas F. E.,
Bentink Stefan,
Döhner Hartmut,
Enz Martina,
Feller Alfred C.,
Hansmann MartinLeo,
Hummel Michael,
Kestler Hans A.,
Klapper Wolfram,
Kreuz Markus,
Lenze Dido,
Loeffler Markus,
Möller Peter,
MüllerHermelink HansKonrad,
Ott German,
Rosolowski Maciej,
Rosenwald Andreas,
Ruf Sandra,
Siebert Reiner,
Spang Rainer,
Stein Harald,
Truemper Lorenz,
Lichter Peter,
Bentz Martin,
Wessendorf Swen
Publication year - 2009
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20617
Subject(s) - cdkn2a , comparative genomic hybridization , biology , follicular lymphoma , gene expression profiling , gene , microarray , gene dosage , genetics , gene expression , microbiology and biotechnology , lymphoma , cancer research , genome , immunology
Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array‐based intermediate‐high resolution genomic profiling in combination with Affymetrix™ gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: −1p36 (18%), +2p15 (24%), −3q (14%), −6q (25%), +7p (19%), +7q (23%), +8q (14%), −9p (16%), −11q (15%), +12q (20%), −13q (11%), −17p (16%), +18p (18%), and +18q (28%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I–II. For this aberration as well as for −6q25 and −6q26, an association with inferior survival was observed. © 2008 Wiley‐Liss, Inc.