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Identification of a novel and myeloid specific role of the leukemia‐associated fusion protein DEK‐NUP214 leading to increased protein synthesis
Author(s) -
Ageberg Malin,
Drott Kristina,
Olofsson Tor,
Gullberg Urban,
Lindmark Anders
Publication year - 2008
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20531
Subject(s) - myeloid leukemia , phosphoprotein , fusion protein , biology , chromosomal translocation , cancer research , microbiology and biotechnology , protein biosynthesis , nucleoporin , transcription factor , phosphorylation , nuclear protein , genetics , gene , recombinant dna
The t(6;9)(p22;q34) chromosomal translocation is found in a subset of patients with acute myeloid leukemia (AML). The translocation results in a fusion between the nuclear phosphoprotein DEK and the nucleoporin NUP214 (previously CAN). The mechanism by which the fusion protein DEK‐NUP214 contributes to leukemia development has not been identified, and disruptions of normal cellular functions by DEK‐NUP214 have previously not been described. In the present study, a novel effect of the DEK‐NUP214 fusion protein is demonstrated. Our findings reveal a substantial increase in global protein synthesis in DEK‐NUP214 expressing cells. Furthermore, we conclude that this effect is not the result of dysregulated transcription but merely due to increased translation. Consistent with the association with AML, the increased protein synthesis mediated by DEK‐NUP214 is restricted to cells of the myeloid lineage. Analysis of potential mechanisms for regulating protein synthesis shows that expression of DEK‐NUP214 correlates to the phosphorylation of the translation initiation protein, EIF4E. The present data provide evidence that increase of translational activity constitutes a mechanism by which the leukemogenic effect of DEK‐NUP124 may be mediated. © 2008 Wiley‐Liss, Inc.