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Genomic and functional evidence for an ARID1A tumor suppressor role
Author(s) -
Huang Jianmin,
Zhao YanLing,
Li Yu,
Fletcher Jonathan A.,
Xiao Sheng
Publication year - 2007
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20459
Subject(s) - arid1a , complementary dna , biology , suppressor , tumor suppressor gene , gene , cancer research , genetics , microbiology and biotechnology , carcinogenesis , mutation
ARID1A is located in 1p36.11, a region frequently deleted in human cancers. Using a novel method to screen for tumorigenic cDNA sequences, we have identified ARID1A as a presumptive tumor suppressor gene. The transforming ARID1A sequence was an antisense cDNA, and was the product of a genomic rearrangement, as corroborated in the primary breast carcinoma from which the cDNA had been obtained. In further screening, we identified a lung adenocarcinoma cell line with a highly localized homozygous genomic deletion involving the 5′ end of ARID1A . These studies provide strong evidence that ARID1A is a tumor suppressor gene. © 2007 Wiley‐Liss, Inc.