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Array comparative genomic hybridization analysis of chromosomal imbalances and their target genes in gastrointestinal stromal tumors
Author(s) -
Ässämäki Reetta,
SarlomoRikala Maarit,
LopezGuerrero José Antonio,
Lasota Jerzy,
Andersson Leif C.,
LlombartBosch Antonio,
Miettinen Markku,
Knuutila Sakari
Publication year - 2007
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20439
Subject(s) - comparative genomic hybridization , pdgfra , biology , cdkn2a , imatinib mesylate , copy number variation , chromosome , cancer research , gene , stromal tumor , copy number analysis , amplicon , genetics , cxcl14 , gist , stromal cell , imatinib , genome , polymerase chain reaction , chemokine receptor , chemokine , immune system , myeloid leukemia
Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. The tumors characteristically harbor KIT or PDGFRA mutations, and mutant tumors respond to imatinib mesylate (Glivec™). Chromosomal imbalances resulting in altered gene dosage are known to have a role in the molecular pathogenesis of these tumors, but the target genes remain to be identified. The present study aimed to identify some of these genes. In total, 35 GIST samples were screened for chromosomal imbalances by array‐based comparative genomic hybridization. A cDNA array was used to define the minimal common overlapping areas of DNA copy number change. Eight confirmative, replicate hybridizations were performed using an oligonucleotide array. The most recurrent copy number losses were localized to 14q, 22q, and 1p. Gains were less common with 8q being the most recurrent. Two recurrent deleted regions of 14q were 14q11.2 harboring the PARP2, APEX1 , and NDRG2 genes and 14q32.33 harboring SIVA . Additional target candidates were NF2 at chromosome 22, CDKN2A/2B at 9p, and ENO1 at 1p for copy number losses, and MYC at 8q for copy number gains. Array CGH proved to be an effective tool for the identification of chromosome regions involved in the development and progression of GISTs. © 2007 Wiley‐Liss, Inc.