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Genomic alterations in lobular neoplasia: A microarray comparative genomic hybridization signature for early neoplastic proliferationin the breast
Author(s) -
Mastracci Teresa L.,
Shadeo Ashleen,
Colby Sarah M.,
Tuck Alan B.,
O'Malley Frances P.,
Bull Shelley B.,
Lam Wan L.,
Andrulis Irene L.
Publication year - 2006
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20368
Subject(s) - lobular carcinoma , comparative genomic hybridization , breast cancer , biology , pathology , tissue microarray , invasive lobular carcinoma , copy number analysis , ductal carcinoma , cancer , copy number variation , genetics , genome , medicine , gene , invasive ductal carcinoma
The identification of genomic alterations occurring in neoplastic lesions provides insight into both lesion occurrence and disease progression. In this study, we used microarray comparative genomic hybridization (CGH) to investigate genetic changes in atypical lobular hyperplasia (ALH) and lobular carcinoma in situ (LCIS), as the presence of these lobular neoplastic lesions is an indicator of risk in the development of invasive breast cancer. DNA was extracted from microdissected archival breast tissue containing ALH or LCIS, lacking adjacent invasive carcinoma, and subjected to whole‐genome tiling path microarray‐CGH using the submegabase resolution tiling set (SMRT)‐array platform. Twelve ALH and 13 LCIS lesions were examined. Copy number alterations were identified using statistical criteria and validated with Real‐Time PCR and fluorescence in situ hybridization. From statistical analysis, a greater number of alterations were observed in ALH compared to LCIS. Alterations common to ALH include gain at 2p11.2 and loss at 7p11–p11.1 and 22q11.1. Alterations common to LCIS include gain at 20q13.13 and loss at 19q13.2–q13.31. In both ALH and LCIS, we observed loss of 16q21–q23.1, an altered region previously identified in lobular neoplasia and invasive carcinoma. The validation of select alterations reinforces the genomic signature. This study represents the first whole‐genome investigation of lobular neoplastic breast lesions using clinical archival specimens. The identified genomic signature includes copy number alterations not previously identified for lobular neoplasia. This genomic signature, common to ALH and LCIS, suggests a role for the acquisition of novel genomic alterations in the aberrant cellular proliferation that defines lobular neoplasia. © 2006 Wiley‐Liss, Inc.