Premium
Contribution of the BOP1 gene, located on 8q24, to colorectal tumorigenesis
Author(s) -
Killian Audrey,
SarafanVasseur Nasrin,
Sesboüé Richard,
Le Pessot Florence,
Blanchard France,
Lamy Aude,
Laurent Michelle,
Flaman JeanMichel,
Frébourg Thierry
Publication year - 2006
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20351
Subject(s) - biology , ribosome biogenesis , carcinogenesis , gene , colorectal cancer , cancer research , genetics , cancer , ribosome , rna
The most common form of genomic instability observed in colorectal cancer is chromosomal instability (CIN), whose molecular bases remain to be determined. We have previously demonstrated that inactivation in human cells of several components of the Pes1‐Bop1 complex ( BOP1, GRWD1, PES1, ORC6L, and RPL3 ), involved in ribosome biogenesis, altered chromosome segregation. To determine the contribution to colorectal tumorigenesis of somatic alterations of genes involved in ribosome biogenesis, we screened 56 primary colorectal cancers, using quantitative multiplex PCR of short fluorescent fragments, a sensitive method for the detection of gene dosage alterations. We found that dosage increase of the BOP1 gene was a frequent event, being detected in 39% of the tumors, and we show that it is associated with an increase of BOP1 mRNA. Scanning of 8q24, on which BOP1 is located, revealed that in colorectal cancers, gene dosage increase of BOP1 can be independent from that of MYC and was more frequent than that affecting MYC . Finally, transient overexpression of BOP1 in human cells increased the percentage of multipolar spindles. Together with our previous results, the present study strongly suggests that deregulation of the BOP1 pathway contributes to colorectal tumorigenesis. © 2006 Wiley‐Liss, Inc.